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Poplar potassium transporters capable of controlling K+ homeostasis and K+-dependent xylogenesis.

Identifieur interne : 004542 ( Main/Exploration ); précédent : 004541; suivant : 004543

Poplar potassium transporters capable of controlling K+ homeostasis and K+-dependent xylogenesis.

Auteurs : Katharina Langer [Allemagne] ; Peter Ache ; Dietmar Geiger ; Andrea Stinzing ; Matthias Arend ; Christa Wind ; Sharon Regan ; Jörg Fromm ; Rainer Hedrich

Source :

RBID : pubmed:12492841

Descripteurs français

English descriptors

Abstract

The cambial K+ content of poplar increases during the growth period in a K+ supply dependent manner. Upon K+ starvation or application of tetraethylammoniumchloride (TEA+), a K+ channel blocker, the average vessel lumen and expansion zone area were significantly reduced. In search for the molecular basis of potassium-dependent xylogenesis in poplar, K+ transporters homologous to those of known function in Arabidopis phloem- and xylem-physiology were isolated from a poplar wood EST library. The expression profile of three distinct K+ channel types and one K+ transporter, Populus tremula K+ uptake transporter 1 (PtKUP1), was analysed by quantitative RT-PCR. Thereby, we found P. tremula outward rectifying K+ channel (PTORK) and P. tremula K+ channel 2 (PTK2) correlated with the seasonal wood production. K+ transporter P. tremula 1 (KPT1) was predominantly found in guard cells. Following the heterologous expression in Xenopus oocytes the biophysical properties of the different channels were determined. PTORK, upon membrane de-polarization mediates potassium release. PTK2 is almost voltage independent, carrying inward K+ flux at hyperpolarized potential and K+ release upon de-polarization. PtKUP1 was expressed in a K+ uptake-deficient Escherichia coli strain, where this K+ transporter rescued K+-dependent growth. In order to link the different K+ transporters to the cambial activity and wood production, we compared the expression profiles to seasonal changes in the K+ content of the bark as well as xylem vessel diameter. Thereby, we found PTORK and PTK2 transcripts to follow the annual K+ variations in poplar branches. PtKUP1 was expressed at a low level throughout the year, suggesting a housekeeping function. From these data, we conclude that K+ channels are involved in the regulation of K+-dependent wood production.

DOI: 10.1046/j.1365-313x.2002.01487.x
PubMed: 12492841


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Le document en format XML

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<term>Cation Transport Proteins (genetics)</term>
<term>Cation Transport Proteins (metabolism)</term>
<term>Cell Division (drug effects)</term>
<term>Cell Division (physiology)</term>
<term>Culture Techniques (MeSH)</term>
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<term>Expressed Sequence Tags (MeSH)</term>
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<term>Gene Expression Regulation, Plant (physiology)</term>
<term>Homeostasis (drug effects)</term>
<term>Membrane Potentials (drug effects)</term>
<term>Membrane Potentials (physiology)</term>
<term>Molecular Sequence Data (MeSH)</term>
<term>Mutation (MeSH)</term>
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<term>Plant Structures (genetics)</term>
<term>Plant Structures (growth & development)</term>
<term>Plant Structures (metabolism)</term>
<term>Populus (cytology)</term>
<term>Populus (genetics)</term>
<term>Populus (metabolism)</term>
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<term>Potassium (pharmacology)</term>
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<term>Potassium Channels (genetics)</term>
<term>Potassium Channels (metabolism)</term>
<term>Potassium Channels (physiology)</term>
<term>Reverse Transcriptase Polymerase Chain Reaction (MeSH)</term>
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<term>Canaux potassiques (métabolisme)</term>
<term>Canaux potassiques (physiologie)</term>
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<term>Division cellulaire (physiologie)</term>
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<term>Ovocytes (physiologie)</term>
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<term>Populus (métabolisme)</term>
<term>Potassium (métabolisme)</term>
<term>Potassium (pharmacologie)</term>
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<term>Régulation de l'expression des gènes végétaux (physiologie)</term>
<term>Saisons (MeSH)</term>
<term>Structures de plante (croissance et développement)</term>
<term>Structures de plante (génétique)</term>
<term>Structures de plante (métabolisme)</term>
<term>Techniques de culture (MeSH)</term>
<term>Transport biologique (MeSH)</term>
<term>Transporteurs de cations (génétique)</term>
<term>Transporteurs de cations (métabolisme)</term>
<term>Tétraéthyl-ammonium (pharmacologie)</term>
<term>Xenopus (MeSH)</term>
<term>Étiquettes de séquences exprimées (MeSH)</term>
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<term>Potassium Channels</term>
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<term>Potassium Channels</term>
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<term>Cation Transport Proteins</term>
<term>Potassium</term>
<term>Potassium Channels</term>
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<term>Structures de plante</term>
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<term>Populus</term>
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<term>Homeostasis</term>
<term>Membrane Potentials</term>
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<term>Canaux potassiques</term>
<term>Division cellulaire</term>
<term>Homéostasie</term>
<term>Potentiels de membrane</term>
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<term>Escherichia coli</term>
<term>Gene Expression Regulation, Plant</term>
<term>Plant Structures</term>
<term>Populus</term>
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<term>Plant Structures</term>
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<term>Canaux potassiques</term>
<term>Escherichia coli</term>
<term>Populus</term>
<term>Régulation de l'expression des gènes végétaux</term>
<term>Structures de plante</term>
<term>Transporteurs de cations</term>
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<term>Populus</term>
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<term>Populus</term>
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<term>Potassium</term>
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<term>Potassium</term>
<term>Potassium Channel Blockers</term>
<term>Tetraethylammonium</term>
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<term>Canaux potassiques</term>
<term>Division cellulaire</term>
<term>Ovocytes</term>
<term>Potentiels de membrane</term>
<term>Régulation de l'expression des gènes végétaux</term>
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<term>Cell Division</term>
<term>Gene Expression Regulation, Plant</term>
<term>Membrane Potentials</term>
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<term>Potassium Channels</term>
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<term>Expressed Sequence Tags</term>
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<term>Xenopus</term>
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<term>RT-PCR</term>
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<term>Techniques de culture</term>
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<term>Xenopus</term>
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<div type="abstract" xml:lang="en">The cambial K+ content of poplar increases during the growth period in a K+ supply dependent manner. Upon K+ starvation or application of tetraethylammoniumchloride (TEA+), a K+ channel blocker, the average vessel lumen and expansion zone area were significantly reduced. In search for the molecular basis of potassium-dependent xylogenesis in poplar, K+ transporters homologous to those of known function in Arabidopis phloem- and xylem-physiology were isolated from a poplar wood EST library. The expression profile of three distinct K+ channel types and one K+ transporter, Populus tremula K+ uptake transporter 1 (PtKUP1), was analysed by quantitative RT-PCR. Thereby, we found P. tremula outward rectifying K+ channel (PTORK) and P. tremula K+ channel 2 (PTK2) correlated with the seasonal wood production. K+ transporter P. tremula 1 (KPT1) was predominantly found in guard cells. Following the heterologous expression in Xenopus oocytes the biophysical properties of the different channels were determined. PTORK, upon membrane de-polarization mediates potassium release. PTK2 is almost voltage independent, carrying inward K+ flux at hyperpolarized potential and K+ release upon de-polarization. PtKUP1 was expressed in a K+ uptake-deficient Escherichia coli strain, where this K+ transporter rescued K+-dependent growth. In order to link the different K+ transporters to the cambial activity and wood production, we compared the expression profiles to seasonal changes in the K+ content of the bark as well as xylem vessel diameter. Thereby, we found PTORK and PTK2 transcripts to follow the annual K+ variations in poplar branches. PtKUP1 was expressed at a low level throughout the year, suggesting a housekeeping function. From these data, we conclude that K+ channels are involved in the regulation of K+-dependent wood production.</div>
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<AbstractText>The cambial K+ content of poplar increases during the growth period in a K+ supply dependent manner. Upon K+ starvation or application of tetraethylammoniumchloride (TEA+), a K+ channel blocker, the average vessel lumen and expansion zone area were significantly reduced. In search for the molecular basis of potassium-dependent xylogenesis in poplar, K+ transporters homologous to those of known function in Arabidopis phloem- and xylem-physiology were isolated from a poplar wood EST library. The expression profile of three distinct K+ channel types and one K+ transporter, Populus tremula K+ uptake transporter 1 (PtKUP1), was analysed by quantitative RT-PCR. Thereby, we found P. tremula outward rectifying K+ channel (PTORK) and P. tremula K+ channel 2 (PTK2) correlated with the seasonal wood production. K+ transporter P. tremula 1 (KPT1) was predominantly found in guard cells. Following the heterologous expression in Xenopus oocytes the biophysical properties of the different channels were determined. PTORK, upon membrane de-polarization mediates potassium release. PTK2 is almost voltage independent, carrying inward K+ flux at hyperpolarized potential and K+ release upon de-polarization. PtKUP1 was expressed in a K+ uptake-deficient Escherichia coli strain, where this K+ transporter rescued K+-dependent growth. In order to link the different K+ transporters to the cambial activity and wood production, we compared the expression profiles to seasonal changes in the K+ content of the bark as well as xylem vessel diameter. Thereby, we found PTORK and PTK2 transcripts to follow the annual K+ variations in poplar branches. PtKUP1 was expressed at a low level throughout the year, suggesting a housekeeping function. From these data, we conclude that K+ channels are involved in the regulation of K+-dependent wood production.</AbstractText>
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